两相体系脂肪酶催化合成蔗糖酯的研究

摘要

In the two-phase solvent mixture formed by amyl alcohol and water,sucrose and vinyl acetate in the free state of lipase are catalyzed under the condition of transesterification reaction;the synthesis of sucrose esters is happened;the synthesis reaction of the various process parameters is determined.When sucrose 1g,the water volume content in 60%,temperature 40 ℃,pH value of buffer solution of 8,lipase dosage is 0.3 g,the ratio of Sucrose and vinyl acetate is 1∶4,reaction time 24 h,sugar conversion rate is 54.8%.Confirmed by IR,the main generated product is 6 sucrose acetate.%研究了正戊醇和水形成的两相体系混合溶剂中,蔗糖和乙酸乙烯酯在游离态脂肪酶的催化作用下,发生转酯化反应合成蔗糖酯,确定了合成反应的各工艺参数。即当蔗糖为1.0g,水体积含量为60%,温度在40℃,缓冲液的pH值为8.0,脂肪酶用量为0.3g,蔗糖与乙酸乙烯酯的比例为1∶4,反应时间24h时,蔗糖的转化率为54.8%。通过红外光谱确定了生成主要的产物是蔗糖-6-乙酸酯。