[目的]观测呼吸道合胞病毒(RSV)毛细支气管炎患儿外周血白细胞5脂氧酶(5-L0)mRNA及5脂氧酶激活蛋白(FLAP)mRNA水平及其与临床严重程度关系。[方法]17例鼻咽分泌物RSV检测阳性的毛细支气管炎患儿为病例组；同期人院的非感染性外科疾病术前患儿10例为对照组，两组年龄分布及性别构成均无显著性差异。采用荧光定量PCR检测两组患儿外周血白细胞5-LO及FLAP mRNA水平，比较两组患儿血白细胞5-LO及FLAP mRNA水平的差异并分析其与临床严重程度的关系。[结果]病例组患儿外周血白细胞5-LO(0.2364±0.0332)和FLAP(0.4106±0.0575)mRNA水平均较对照组(分别为0.0018±0.0013和0.0044±0.0030)明显升高，P值均＜0.01。病例组患儿外周血白细胞5-LO和FLAP mRNA水平与临床严重程度评分均呈正相关(r分别为0.745，0.594，P值均＜0.05)。[结论]RSV毛细支气管炎患儿外周血白细胞5-LO mRNA及FLAP mRNA水平升高并与临床严重程度呈正相关。%[Objective] To observe 5-lipoxygenase (5-LO) mRNA level and 5-lipoxygenase-Activating protein (FLAP) mRNA level of the peripheral blood white blood cells (WBC) and their correlation to the clinical manifestation in RSV bronchiolitis children. [Methods] Seventeen children suffered from bronchiolitis whose nasopharyngeal secretion positive for RSV-Ag were put into patient group. 10 preoperative patients with matched age, gender proportion and no evidence of infection were chosen as control group. 5-LO mRNA level and FLAP mRNA level in peripheral blood WBC were measured by FQ Reverse Transcription- Polymerase Chain Reaction (RT-PCR) in both groups. Severity of RSV bronchiolitis was assessed of by respiratory disease assess instrument (RDAI) and the correlation between 5-LO/FLAP mRNA levels and the severity of RSV bronchiolitis were analyzed. [Results] The mRNA levels of 5-LO [(0.2364 ± 0.0332) vs (0.0018 ± 0.0013)]and FLAP [(0.4106 ± 0.0575) vs (0.0044 ± 0.0030)]were significantly higher than that in control group(P < 0.01). Both the 5-LO mRNA level and the FLAP mRNA level were positively related to the severity of RSV bronchiolitis (R = 0.745, 0.594, respectively,P < 0.05 both). [Conclusion] The mRNA levels of 5-LO and FLAP in peripheral blood WBC in RSV bronchiolitis children are elevated and positively related to the severity of RSV bronchiolitis.
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