STAT4基因rs10181656位点的多态性与不明原因复发性自然流产的相关性

摘要

[目的]探讨信号转导与转录激活因子4(STAT4)基因在不明原因复发性自然流产(URSA)表达及其表达量与rs10181656位点多态性的关系.[方法]分别采用聚合酶链反应-限制性片段长度多态性法检测332名URSA患者和260名健康对照者rs10181656位点的基因型频率;应用免疫组织化学技术检测其中的86名URSA患者及77名健康对照者蜕膜STAT4基因的蛋白表达量.[结果]rs10181656C/G:URSA组中C/C、C/G及G/G基因型频率分别是36.45％、46.99％及16.57％,而对照组中的3种基因型的频率分别是46.54％、45.38％及8.08％,具有统计学差异(P＜0.05),G等位基因增加URSA组发病风险(OR=1.50,P＜0.05).STAT4蛋白表达量在URSA组和对照组中的差异具有统计学意义(P＜0.05);在URSA组内分为C/C、C/G及G/G基因型的三个亚组STAT4蛋白表达:三个亚组的STAT4蛋白表达差异具有统计学意义(P＜0.05),在对照组中亦然(P＜0.05),具有G/G基因型样本STAT4蛋白高表达于C/C基因型(P均＜0.05);相同基因型在URSA组和对照组中STAT4蛋白表达:C/C基因型样本在URSA组和对照组中STAT4蛋白的表达差异无统计学意义(P＞0.05),C/G及G/G基因型样本亦然(P＞0.05).[结论]rs10181656位点多态性通过影响STAT4蛋白表达,从而与增加URSA发病风险相关.%[Objective] To investigate associations between the functional polymorphisms of signal transducer and activator of transcription 4 (STAT4) gene and unexplained recurrent spontaneous abortion (URSA) and between STAT4 protein expression and the genotypes of rs 10181656 locus.[Methods] PCR-restriction fragment length polymorphism was used to genotype rs 1 0181656 locus polymorphism in 332 URSA cases and 260 normal controls,in 86 URSA cases and 77 normal controls of which immunohistochemical technique was used to detect STAT4 protein expression.[Results] The frequencies of rs10181656 C/G were 36.45 ％,46.54％ in genotype C/C,46.99％,45.38％ in genotype C/G and 16.57％,8.08％ in genotype G/G between URSA patients and normal controls.They reached statistical difference (P ＜ 0.05).The carriers of rs 10181656 G allele increased the risk of URSA (OR =1.50,P ＜ 0.05).STAT4 protein expression in decidual tissues:①There was statistical difference in the STAT4 protein expression in decidual tissues between cases and controls (P ＜ 0.05).In URSA patients there was statistical difference in the STAT4 protein expression among genotype CC,CG and GG of rs10181656 locus (P ＜ 0.05).So was in normal controls (P ＜ 0.05).In genotype CC there was no difference in the STAT4 protein expression between cases and controls (P ＞ 0.05).Neither was In genotype CG and GG respectively (P all ＞ 0.05).[Conclusion] Functional polymorphisms of the rs10181656 locus could probably associate with the susceptibility of URSA via STAT4 protein expression increased by genotype G/G in maternal decidual tissue.