大花细辛叶提取物对小鼠热板致痛的镇痛作用

摘要

Objective: To study analgesic effect of the ethanol extracts from the leaves of Asarum maximum Hemsl on mice and its active fractions. Method: The five fractions were obtained from the extractor, extracted by 95% ethanol from the leaves of Asarum maximum Hemsl by systematic solvent extraction method respectively using petroleum ether, chloroform, acetic ether, n-butanol and water. Mice pain model was induced by hot plate. Result: The extracts of 95% ethanol (500 (mg ? Kg" )) from the leaves of Asarum maximum Hemsl could increase the threshold of pain on mice induced by hot plate. The effect of the n-butanol fraction (500 (mg ? Kg"1) ) increasing the threshold of pain notably on mice is better than that of Aspirin (200 (mg ? Kg"1)). Conclusion: The ethanol extracts from the leaves of Asarum maximum Hemsl have analgesic effects, and the n-butanol fraction is the main active fraction.%目的：研究大花细辛叶乙醇提取物的镇痛活性及其镇痛活性组分。方法：通过小鼠热板法，研究大花细辛叶的乙醇提取物以及采用系统溶剂法分离得到的5个组分的镇痛活性。结果：大花细辛叶95％乙醇提取物(500 mg/kg)能提高小鼠痛阈值，正丁醇萃取部分(500 mg/kg)能显著提高痛阈值且优于对照组阿司匹林(200 mg/kg)。结论：大花细辛叶的乙醇提取物有镇痛作用，正丁醇萃取部分是镇痛的主要组分。