大胡蜂窝对酸性乙醇诱导的大鼠胃溃疡的保护作用和抗氧化活性

摘要

Objective To examine the protective effects of wasp (Vespa magnifica)honeycomb extract(WCE)against gastric lesions in rats induced by 60% acidified ethanol,and evaluate its capacity to suppress oxidative stress in the gastric tissue.Methods Wistar rats were subiected to intragastric administration of 60% acidified ethanol to induce gastric lesions following an 8-day oral pretreatment with WCE at 0,25,100 and 150 ms/kg or with saline.The levels of 1,1-dipheny1-2-picrylhydrazyl(DPPH)free radical scavenging,myeloperoxidase (MPO) activity and total antioxidant capacity in the gastric tissues were determined.Results Oral administration of 25,100 and 150 rag/kg WCE prior to 60% acidified ethanol administration significantly inhibited the formation of gastric lesions (with a reduction by 44.2%-87.1%),decreased the mucosal MPO activity(by 16.4%-56.6%)and increased the total antioxidant capacity of the gastric tissue(by 0.5,1.47 and 1.83 folds,respectively)in a dose-dependent manner.At a high concentration (above 1 mg/mi),WCE also exhibited a stronger DPPH radical scavenging activity than butylated hydroxytoluene (BHT).Conclusion The ethanol extract of wasp honeycombs can suppress the formation of acidified ethanol-induced gastric lesions by reducing free radical oxidation and neutrophils infiltration in the gastric tissue in rats.%目的 研究大胡蜂窝乙醇抽提物(WCE)对60%酸性乙醇诱导的大鼠胃溃疡的保护性及其作用机制.方法 用WCE按照0、25、100和150mg/kg的剂量对Wistar大鼠连续灌胃8 d,然后用60%的酸性乙醇诱导胃溃疡,比较不同处理组大鼠胃粘膜的病变程度、髓过氧物酶活性和总抗氧化能力,同时分析WCE体外抗二苯基三硝基肼自由基的能力.结果 与0mg/ks WCE灌胄组相比,25、100和150 mg/kg WCE灌胃组的胃溃疡程度下降了44.2%～87.1%;胃粘膜髓过氧物酶活性下降了16.4%～56.6%,胃粘膜的总抗氧化能力分别增加0.5、1.47和1.83倍.此外,WCE体外自由基清除能力显著高于二丁基羟基甲苯.结论 通过降低胃粘膜自由基的破坏性和中性粒细胞的渗漏性,WCE能显著抑制60%酸性乙醇诱导的大鼠胃溃疡和溃疡形成过程中的氧化反应.