腹腔镜下猪胆漏动物模型的建立

摘要

Objective To establish a animal model of biliary leakage in pigs with laparoscopy. Methods Eight healthy Bama minipigs were subject to laparoscopic surgery under general anesthesia.The cystic duct was resected at 0.5-1.0 cm from the root and the stump was left open with the gallbladder removed. Blood routine and hepatic functions of the pigs were tested before and 24 h after the surgery, and endoscopic retrograde cholangiography (ERC) was performed 24 h after the surgery. At 48 h after the surgery, the pigs were sacrificed for observation of the stump of the cystic duct. Results The gallbladder, cystic duct, cystic artery, and the anatomical relations between the gallbladder and liver and between the cystic duct and common bile duct were fully exposed under laparoscopy. White blood cells, neutrophils and direct bilirubin increased significantly after the operation (FO.05). The cystic ducts were resected at 0.5-1 cm away from the roots of the cystic ducts in 7 pigs and at 2 cm in 1 pig. Conclusion Laparoscopy is safe and feasible for establishing the porcine model of biliary leakage.%目的 探讨腹腔镜下建立猪胆漏动物模型可行性.方法 8只健康巴马小型猪为研究对象,全麻下行腹腔镜手术,距胆囊管根部0.5～1.0 cm处离断胆囊管,胆囊管残端开放,剥离切除胆囊；通过术前、术后24 h监测血常规、肝功；术后24 h行内镜下逆行胆管造影(ERC)；死亡动物及时解剖,存活动物术后48 h处死解剖观察胆囊管残端.结果 腹腔镜下能充分暴露猪胆囊、胆囊管、胆囊动脉,显示胆囊与肝脏及胆囊管与胆总管的解剖关系.与术前相比,术后24 h白细胞、中性粒细胞、直接胆红素明显升高(P＜0.05).8只实验猪7只距胆囊管根部0.5～1.0 cm处离断胆囊管,1只距胆囊管根部约2 cm处离断胆囊管.结论 腹腔镜下制备猪胆漏动物模型安全、可行,微创.