[目的]提高姜黄素生物利用度,增加稳定性,促进姜黄素在临床上的应用.[方法]采用乙醇注入法制备姜黄素脂质体,通过单因素试验和正交试验,以包封率为主要指标,优选最佳制备工艺,并观察脂质体形态和粒径特征.[结果]乙醇注入法制备姜黄素脂质体的最佳工艺为:姜黄素用量1.0 mg,胆固醇一卵磷脂比例1∶3,磷酸盐缓冲液(PBS) pH 6.5、用量20.0 mL.制得的姜黄素脂质体包封率为72.32％,平均粒径830 nm左右.[结论]采用乙醇注入法制备姜黄素脂质体,工艺简单,易于掌握,且包封率、稳定性较高.%[Objective]The present experiment was conducted to enhance the bioavailability and stability of curcumin in order to improve its clinical application. [ Method ] Lipsomal in curcumin was prepared by using ethanol injection method. The liposome encapsulation rate was used to screen the best processing using single factor orthogonal design. The shape characteristics and particle size of lipsomal in curcumin were investigated. [Result]The best optimization technology for preparation of lipsomal in curcumin was as follows: 1.0 mg of curcumin, 1:3 ratio of lecithin to cholesterol and 20.0 mL phosphate buffer salt solution (pH 6.5). With this method, the encapsulation rate of curcumin liposome was recorded as 72.32% and the particle size was about 830 nm. [Conclusion ]The developed method for preparation of liposomal in curcumin using ethanol injection was simple and easy to control. The encapsulation rate and stability were very high.
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