乙醇注入法制备木犀草素脂质体

摘要

探讨乙醇注入法制备木犀草素脂质体的制备工艺．采用乙醇注入法制备木犀草素脂质体，微柱离心和HPLC法测定包封率，激光粒度仪测定粒径．运用正交实验设计L9（34）优选最佳处方为：磷脂600 mg，药脂比1∶7，类酯比8∶1，有机相水相比1∶1，包封率79．3％，形态，稳定性良好．注入法制备木犀草素脂质体工艺简单，制得剂型稳定，方法可行．%To investigate the preparation of ethanol injection of luteolin liposome , the lipo-some was prepared by ethanol injection .Entrapment rate was measured by microcolumn off-center and HPLC .The particle size of luteolin liposome was measured by using the laser par-ticle size analyzer.Orthognal test L9(34)was applied to choose the best method, and the re-sult was as follows:PC (600 mg), PC∶Cho (6∶1), cho∶PC (1∶9), ethanol∶PBS (1∶1), entrapment rate of 79.3%.The shape and stability were good .The method of ethanol injec-tion of luteolin liposome was easy , and the dosage was stable .So this method could be ap-plied in luteolin liposome preparation .