进行香蕉假茎生物脱胶菌株筛选和生物脱胶提取香蕉假茎纤维初步试验研究,为生物脱胶提取香蕉假茎纤维实际应用提供依据.从腐烂香蕉假茎和土壤等样品中分离筛选对香蕉假茎具有脱胶作用菌株,并利用筛选出优良菌株对香蕉假茎进行脱胶,测定假茎失重率、纤维得率和残胶率,对菌株脱胶性能进行综合评价.筛选出FR1和BR2-1菌株对香蕉假茎均表现出很好脱胶效,其最佳脱胶时间均为72 h,脱胶前香蕉假茎含胶率为83.6％,脱胶后分别降至24.7％和20.2％,失重率分别达72.9％和77.8％,纤维得率分别达2.27％和2.01％.筛选获得FR1和BR2-1菌株在香蕉假茎脱胶提取纤维上均有较好应用潜力,表明应用生物脱胶去除香蕉假茎中胶质物质来提取生物纤维完全可行.%[Objective]Preliminary studies on bio-retting of false stem of banana (Musa nana Lout) were carried out using microbial strains in order to provide basis for the practical application of bio-retting technique in extracting fibers from false stem of banana. [Method]Microbial strains for bio-retting were isolated from false stem of rotten banana plants and soil. The strains were screened and superior ones were used in the bio-retting experiment. Their bio-retting performance was assessed comprehensively by determining false stem weight-loss ratio, fiber yield and the rate of residual gum in fiber. [Result]Two strains, viz., FR1 and BR2-1, having good ability for bio-retting false stem of banana, were screened out. The time for bio-retting of stem taken by these strains was found to be 72 h. The rates of gum in false stem, bio-retted by FR1 and BR2-1, decreased rapidly from 83.6 to 24.7 and 20.2% , respectively. The weight-loss ratio of false stem reached 72.9 and 77.8%, and the yields of fiber recorded as 2.27 and 2.01%, after bio-retting of false stem by strains FR1 and BR2-1, respectively. [Conclusion]The screened microbial strains FR1 and BR2-1 showed good application potential for bio-retting of banana false stem, therefore, may be used for bio-retting of banana false stem for the removal of gum and extraction of fibers.
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