红罗非鱼稚鱼与雌雄亲鱼肌肉脂肪酸组成分析

摘要

[目的]测定红罗非鱼(Oreochromis sp.)稚鱼、雌雄亲鱼的肌肉脂肪酸组成,为红罗非鱼的生物学研究及科学养殖提供基础资料.[方法]采用常规的化学分析方法及气相色谱技术检测红罗非鱼稚鱼与雌雄亲鱼肌肉脂肪酸的组成及含量变化.[结果]从红罗非鱼稚鱼肌肉中检测出10种脂肪酸,雌雄亲鱼肌肉中检测出16种脂肪酸,以C16∶0、C16∶1、C18∶0、C18∶1n-9、C18∶2n-6的含量较高,占总脂肪酸含量的84％以上.红罗非鱼肌肉脂肪酸含量因生长期和性别不同而异,C20∶5n-3(EPA)和C22∶6n-3(DHA)在稚鱼肌肉中未能检出,雌亲鱼肌肉中含有少量(0.47％h和0.65％),雄亲鱼肌肉含量丰富(1.39％和2.33％)；红罗非鱼稚鱼和雄性亲鱼肌肉中,脂肪酸含量次序为:饱和脂肪酸含量(∑SFA)＞单不饱和脂肪酸含量(∑MUFA)＞多不饱和脂肪酸含量(∑PUFA),雌性亲鱼肌肉中:∑MUFA＞∑SFA＞∑PUFA.[结论]红罗非鱼稚鱼阶段主要依靠∑SFA提供能量以满足其生长所需,雌雄亲鱼则依靠∑MUFA和∑PUFA来提高其繁殖性能.%[Objective]Fatty acid compositions in muscle of fry,male and female parent fish (Oreochromis sp.) were determined to provide references for biological research and scientific breeding of Oreochromis sp..[Method]The fatty acid composition and content in muscle of fry,female and male parent fish were analyzed using conventional chemical method and GC technology.[Result]The results showed that there were ten kinds of fatty acid in muscle of fry,and sixteen kinds of fatty acid in muscle of parent fish.C16∶0,C16∶1,C18∶0,C18∶1n-9 and C18∶2n-6 (ARA) took up more than 84％ of total fatty acid.Fatty acid content in muscle of red tilapia was affected by different growth stages and genders.C20∶5n-3 (EPA) and C22∶6n-3 (DHA) were not found in muscle of fry fish,but little in female parent fish (0.47％ and 0.65％),and much in male parent fish (1.39％ and 2.33％).Fatty acid content showed the following descending sequence of ∑SFA＞ ∑MUFA＞∑PUFA in muscle of fry red tilapia and male parent fish,and ∑MUFA＞∑SFA＞∑PUFA in muscle of red tilapia female parent fish.[Conclusion]Fry red tilapia mainly lives on ∑SFA,while male and female parent fish need ∑MUFA and ∑PUFA to improve reproductive performance.