Objective: To explore the expression of BTG1 in renal clear cell carcinoma ( RCCC ) and its role in 786-O cells proliferation and apoptosis in vitro. Methods : The expression of BTG1 in 20 RCCC specimens was examined using immunohistochemical staining, the BTG1 expression plasmid was constructed and BTG1 siRNA was designed, followed by respective transfer into 786-O cells. Observation on its role in cell proliferation and apoptosis was performed with MTT and Hoechst respectively. Results :Immunohistochemistry demonstrated 85％ ( 17/20 )positive BTG1 staining in malignant kidney tissue of 20 patients, compared to 25％ ( 5/20 ) of normal adjacent tissue ( P ＜0. 05 ). Overexpression of BTG1 in 786-O cells reduced their proliferation but induced their apoptosis, while knockdown BTG1 in 786-O cells increased their proliferation but reduced their apoptosis( P ＜ 0. 05 ). Conclusion :BTG1 may be involved in RCCC carcinogenesis.%目的:探讨B细胞易位基因1(BTG1)在肾透明细胞癌组织中的表达及对肾透明细胞癌细胞株786-O增殖和凋亡的影响.方法:采用免疫组化法检测20例肾透明细胞癌及其癌旁组织中BTG1蛋白的表达.构建BTG1表达质粒及合成BTG1的干扰RNA分别转染786-O细胞,通过MTT法实验观察BTG1对786-O细胞体外增殖的影响,Hoechst染色观察BTG1对786-O细胞凋亡的影响.结果:20例肾透明细胞癌组织中,BTG1蛋白阳性表达5例,阳性率25%;对应的癌旁组织中,BTG1蛋白阳性表达17例,阳性率85%.MTT和Hoechst染色实验分别提示在786-O细胞中高表达BTG1能够抑制其增殖、促进凋亡,在786-O细胞中低表达BTG1能够促进其增殖、抑制凋亡(P＜0.05).结论:在肾透明细胞癌中,BTG1行使抑癌基因的功能,与肾透明细胞癌的发生、发展有关.
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