zebularine对卵巢癌细胞的生长抑制作用

摘要

目的:观察去甲基化制剂zebularine(Zeb)对体外培养的卵巢癌细胞A2780的生长抑制作用,并进一步探讨其作用机制.方法:应用不同浓度(50、100、200 μmol·L-1) 的Zeb处理体外培养的卵巢癌细胞A2780;应用MTT法检测细胞增殖活性,流式细胞仪检测细胞周期分布,Annexin V-FITC染色法检测细胞凋亡率.结果:100、200 μmol·L-1的Zeb处理卵巢癌细胞A2780 24～72 h后,细胞增殖显著受到抑制(P＜0.05);100、200 μmol·L-1的Zeb处理72 h后A2780细胞G2期细胞数量显著增加(P＜0.05),S期细胞数显著减少(P＜0.05);100、200 μmol·L-1组细胞凋亡率均增加(P＜0.05).结论:Zeb对卵巢癌细胞A2780具有生长抑制作用,其机制与阻滞细胞周期及诱导凋亡有关.%Objective: To investigate the effect of zebularine( Zeb ), one of the demethylating agents, on the proliferation of human ovarian cancer cell line A2780 and its mechanism. Methods: A2780 cells cultured in RPMI 1640 were repectively treated with 50 , 100 . and 200 μmol·L-1 of Zeb. MTT assay , flow cytometry and Annexin VFITC staining were performed to analyze the cell proliferation activity, cell cycle and apoptotic rate. Results : After A2780 cells were treated with Zeb of 100 and 200 μmol·L-1 for 24 to 72 hours, the cell proliferation activity was inhibited( P ＜0. 05 ). The number of cells in G2 phase increased and the number of cells in S phase decreased significantly after the cells were treated with Zeb of 100 and 200 μmol·L-1 for 72 hours( P ＜0. 05 ). The apoptotic rate increased significantly in cells treated with Zeb of 100 and 200 μmol·L-1( P ＜ 0. 05 ). Conclusion : Zeb could inhibit the growth of human ovarian cancer cell A2780, possibly via blocking cell cycle and inducing apoptosis.