肿瘤坏死相关凋亡诱导配体对前列腺癌及膀胱癌细胞增殖、凋亡的作用

摘要

目的：研究重组并纯化的肿瘤坏死因子凋亡诱导配体（ TRAIL）蛋白对前列腺癌及膀胱癌细胞增殖及凋亡的影响。方法：将不同浓度的TRAIL蛋白与培养的前列腺癌细胞（ PC-3细胞）和膀胱癌细胞（5637细胞）共孵育24 h后，采用细胞增殖实验及流式细胞术检测肿瘤细胞的增殖及凋亡。结果：PC-3细胞株中，与未处理组比较，TRAIL蛋白浓度在20、40、80、160 ng · ml －1时细胞增殖率均有显著性差异；而在5637细胞株中，与未处理组比较，TRAIL蛋白浓度在5、10、20、40 ng · ml －1时细胞增殖率均有显著性差异。流式细胞实验结果也证实，随TRAIL蛋白浓度的增高，两种肿瘤细胞凋亡率增加。结论：重组并纯化TRAIL蛋白可通过抑制前列腺癌及膀胱癌细胞的增殖，诱导其凋亡而产生抗肿瘤的作用。%Objective: To study the effect of purified tumor necrosis factor-related apoptosis ligand on the proliferation and apoptosis of prostate cancer cell (PC-3 cell) and bladder cancer cell(5637 cell).Methods:PC-3 and 5637 cell line were incubated with different concentrations of TRAIL protein for 24 h.Then cell proliferation and flow cytometry were employed to detect the PC-3 and 5637 proliferation and apoptosis .Results:The significant difference of the cell proliferation rate was found in PC-3 cells when the TRAIL protein concentration was 20,40, 80, 160 ng · ml-1 compared with untreated group .In 5637 cells, the cell proliferation rate had significant difference when the TRAIL protein concentration was 5, 10, 20, 40 ng · ml -1 compared with untreated group . Flow cytometry results also confirmed that the apoptosis rate of two kinds of tumor cell increased with the TRAIL protein dose dependently .Conclusion:Purified tumor necrosis factor-related apoptosis ligand protein can perform the anti-tumor role by inhibiting proliferation and inducing the apoptosis of the prostate cancer and bladder cancer cells .