黄瓜抗枯萎病离体鉴定技术的研究

摘要

Influence of two kinds of selection agents on the tissue cultureof ten genotypes with different resistance to fusarium wilt in cucumber were studied .The results showed that the suitable agents of in vitro evaluation were the culture filtrates of Fusarium oxysporum f.sp. cucumerinum(FOC)and 1 mg/L crude fusaric acid (FA )extracts ，and the suitable period for identification was the induction of callus phase .The tissue culture resistance to agents showed consistence with the seedling resistance to the pathogen .%通过比较10种具有不同枯萎病抗性的黄瓜材料对两类选择压力的反应，确定离体抗性鉴定的适宜选择压力为FOC（the culturefiltrates of Fusarium oxysporum f.sp. cucumerinum）原液和1mg/LFA（fusaric acid）粗制品溶液，适宜的鉴定时间为愈伤组织诱导期.黄瓜材料离体培养物对选择压力的反应与材料苗期抗病性比较一致.