中国3种不同生物型烟粉虱的种群DNA多态性研究

摘要

利用基因组DNA的多态性研究了中国3种不同生物型烟粉虱的种群分化. 烟粉虱种群分别采自北京、扬州和广州的不同寄主上,烟粉虱生物型包括B型、本地型(NaC)和一种新的生物型(CV型). 对3种生物型烟粉虱的RAPD-PCR结果进行遗传距离聚类分析,结果显示烟粉虱中国本地型和CV型的种群关系比较近缘,二者与B型种群的亲缘关系较远. 3个烟粉虱生物型的种群分化属于种内分化,据此推测CV型烟粉虱可能起源于中国或其临近地区.%The differentiation of three haplotypes of Bemisia tabaci populations in China was analyzed using RAPD-PCR in this study. Five B. tabaci populations were collected in the north, middle and south of China, respectively, including three B biotype populations, the indigenous haplotype populations (NaC haplotype) and a new haplotype population from ornamental plants (Cv haplotype). In addition, the greenhouse whitefly, Trialeurodes vaporariorum, was used as a outgroup population in this analysis. The results of the cluster analysis using genetic distances indicated that, first, the population relationship between the NaC haplotype and the Cv haplotype was more close than that between B biotype and NaC haplotype, or that between B biotype and the Cv haplotype; second, the population relationships of the three haplotype of B. tabaci in China was intraspecific, and the Cv haplotype was potentially originated in China or the neighboring countries.