三化螟对海芋挥发油的嗅觉和触角电位反应

摘要

在实验室条件下用四臂嗅觉仪和触角电位(EAG)仪测定了三化螟Scirpophaga incertulas(Walker)对海芋Alocasia macrorrhiza(L.)挥发油的嗅觉反应和触角电位.结果表明,三化螟对海芋挥发油极为敏感,具明显的驱避作用,并能激发三化螟触角产生显著EAG反应.在相同浓度条件下,雌虫的EAG值显著大于雄虫,表明雌虫对植物挥发性物质反应较雄虫强烈.左右触角对挥发油EAG反应并不一致,低浓度条件下[即V(挥发油):V(石蜡)=1:16或1:8],左、右触角的EAG反应差异不显著,但在高浓度条件下[即V(挥发油):V(石蜡)=1:4、1:2或1:1],右触角的EAG反应则显著大于左触角.%The four-armed olfactometer and eletroantennogram (EAG) were used respectively to measure the olfactory and EAG response of the yellow stem borer, Scirpophaga incenulas( Walker), adults to the volatiles from Alocasia macrorrhiza( L. ) in laboratory. The results showed that the pest was extremely sensitive to the volatiles with a clear repellent effect and there were significant EAG responses to the volatiles. Olfactory and EAG response indicated that the adults of the yellow stem borer had negative response to the volatiles derived from A. macrorrhiza. EAG response of female adults was more sensitive than males under the same concentration of volatiles. EAG response of antennae was no significant differences under the conditions of low concentration [ V(volatile): V(olefin) = 1:16 or 1:8 ], but at high concentrations [ V(volatile): V(olefin) = 1: 4,1: 2 or 1: 1 ], the EAG response of the right antenna was significantly more sensitive than the left antenna.