The morphological characters of compound eyes dysplasia in Drosophilia melanogaster induced by azadirachtin was investigated. Flies were allowed to lay eggs on food mixture with 2 mg/kg of azadirachtin on which the resultant larvae and adults were fed and sampled for observation at different developmental stages. The images of stereo microscope and scanning electron microscope showed that adult eyes treated with 2 mg/kg of azadirachtin became smaller in size, irregular in ommatidia organization, rough on surface, duplicated, bent and forked in interommatidial bristles. The ultrastructure of compound eyes was characterized by transmission electron microscope. Results showed that chromatin condensation, phagocytic vacuole increase, nuclear membrane shrinkage, boundary fuzziness occurred in eye cells at the late third instar larvae, and they also showed that photoreceptors loss in different degrees at pupae and new adults. These results suggested that azadirachtin could induce abnormal morphological development in Z). melanogaster compound eyes, which may gain a new insight into novel insect growth regulators.%将果蝇置于含印楝素2mg/kg的昆虫培养基中产卵,待卵孵化发育至不同虫态,运用光学显微镜及扫描电镜研究果蝇新羽化成虫复眼外观,发现复眼明显萎缩并呈现不规则状,表面粗糙,出现异常凹陷,刚毛缺失或增生,排列不规则或弯曲;运用透射电镜研究复眼超微结构,结果表明,3龄末期幼虫复眼组织细胞中染色质高度聚集成小体、小体分散,吞噬泡数量明显增多,核膜皱缩、界限模糊,蛹和新羽化成虫复眼中感光细胞丢失.这些结果确证了印楝素诱导果蝇复眼发育异常,为寻找新型昆虫生长发育调节剂提供了可能的新靶标.
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