荔枝LEAFY同源基因克隆及表达分析

摘要

A litchi LEAFY homologue (LcLFY) cDNA in full length was isolated from floral buds in 'Nuomici' litchi, Litchi chinensis, using RT-PCR and RACE-PCR. The full length of the cDNA was 1 397 bp, containing an open reading frame of 1 171 bp, which encoded 390 amino acids. Furthermore, RT-PCR analysis revealed that LcLFY was expressed during floral differentiation. LcLFY mRNA began to accumulate in buds in the early stage of floral differentiation, and it increased in floral primordium formation stage. However, the LcLFY transcription level decreased in flower formation stage.%采用RT-PCR与RACE-PCR相结合的方法,从‘糯米糍’荔枝Litchi chinensis花芽中分离得到了‘糯米糍’荔枝LEAFY同源基因(LcLFY)的cDNA全长序列.基因全长1 397 bp,其中编码区1 171个碱基,推测编码390个氨基酸.采用半定量PCR技术研究了LFY同源基因在荔枝花芽分化进程的表达情况.结果表明,在‘糯米糍’荔枝花芽分化开始时检测到LFY同源基因的表达,在花序原基出现前后表达增强,但在花分化的阶段表达水平明显减弱.