[Objective]The residue depletion of lincomycin hydrochloride-spectinomycin sulfate injection in pigs was investigated in this study , and its withdrawal time was estimated .[Method] In routine breed-ing conditions , thirty healthy pigs were randomly allocated to six treatment groups .All of them were trea-ted with lincomycin hydrochloride-spectinomycin sulfate injection at a dosage of 15 mg/kg ( 5 mg linco-mycin and 10 mg spectinomycin ) once daily for 5 consecutive days .Five medicated pigs were slaughtered at 8, 24, 72, 144, 240 and 336 h after intramuscular administration, and muscle, liver, kidney, fat and injection site tissues were sampled .The tissue concentration of lincomycin and spectinomycin was ana-lyzed respectively by liquid chromatography-mass spsectrometry ( LC-MS/MS) and high-performance liq-uid chromatography ( HPLC ) .[Result and conclusion] At 6 days postmedication , all observations of spectinomycin and lincomycin were below the corresponding MRLs .In all of the detected tissues , kidney had the highest drug concentration followed by liver , injection site , muscle and fat with the lowest drug concentration , indicating that kidney was target tissue for spectinomycin and lincomycin .Based on the MRLs, which were established by the Chinese Agriculture Ministry , the withdrawal time in pigs was ana-lyzed by WT1.4 software.The results indicated that the withdrawal time of lincomycin hydrochloride-spectinomycin sulfate injection should be 8 days after intramuscular administration of 15 mg/kg in pigs.%[目的]对一种盐酸林可霉素-硫酸大观霉素注射液在猪组织中的残留消除进行研究，并制订该制剂在猪体内的休药期．[方法]常规饲养条件下，将30头健康猪随机分为6组，按猪体质量以15 mg/kg （即林可霉素5 mg/kg和大观霉素10 mg/kg）剂量颈部肌内注射盐酸林可霉素-硫酸大观霉素注射液，每天1次，连用5 d，于最后一次给药后8、24、72、144、240、336 h各宰杀1组猪，分别采集每头猪的肌肉、肝脏、肾脏、脂肪和注射位点5种组织，采用LC-MS/MS法和HPLC-UV法分别对林可霉素和大观霉素进行残留量测定．[结果和结论]林可霉素在猪组织中消除较快，大观霉素消除较慢，给药后第6天所有检测组织中的林可霉素和大观霉素已全部低于最高残留限量，林可霉素和大观霉素在猪体内各组织器官的残留浓度大小顺序均为：肾脏＞肝脏＞注射部位＞肌肉＞脂肪，表明肾脏是二者的靶组织．结合农业部规定的林可霉素、大观霉素在猪体内的最高残留限量和WT1.4休药期软件计算结果，建议该制剂在猪体内的休药期为8 d．
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