新西兰白兔中Myh6基因的表达及与肌纤维性状的相关性

摘要

【目的】分析Myh6基因在新西兰白兔Oryctolagus cuniculus肌肉中的表达规律及与肌纤维特性的相关性，探究Myh6基因在肌纤维发育过程中的作用。【方法】利用冰冻切片技术测量了2、4、6、8、10、12周龄新西兰白兔背腿肌中不同类型肌纤维的密度、面积、长径和短径等性状，采用qRT-PCR测量了Myh6基因在不同周龄新西兰白兔背腿肌中的表达量，分析了上述性状与Myh6基因表达的相关性。【结果】2～8周龄新西兰白兔各类型肌纤维的密度逐渐变小，面积、长径和短径则逐渐变大（白肌纤维短径除外，仅在2～6周龄有变大趋势）。 Myh6基因在4周龄表达量最高。 Myh6基因表达量与公兔背部红肌纤维的面积呈极显著负相关（P＜0.01），与公兔腿部红肌纤维的密度呈极显著正相关（ P＜0.01）。【结论】Myh6基因表达与肌纤维类型和肌纤维的生长发育密切相关。%Objective] To analyze Myh6 gene expression and its correlation with the muscle fiber traits of New Zealand white rabbits , and explore the role of Myh6 gene during the development of muscle fibers.[Method]Density, area, long and short diameters for back and leg muscle fibers were measured for 2-, 4-, 6-, 8-, 10-and 12-week-old New Zealand white rabbits using the frozen section technique .Myh6 gene expressions in back and leg muscles of rabbits at different ages were estimated using qRT-PCR.The correlations between Myh6 gene expression and muscle traits were analyzed .[Result] The density of various muscle fibers gradually decreased , but the area , long and short diameters of muscle fibers increased from two to eight weeks , except for the short diameters of white muscle fibers increased from two to six weeks .The expression level of Myh6 gene was the highest in 4-week-old rabbit .The expression level of Myh6 gene was significantly ( P<0.01 ) negatively correlated with the red muscle fiber area of male rabbit back muscles , and significantly ( P<0.01 ) positively correlated with the red muscle fiber density of male rabbit leg muscles .[Conclusion] Myh6 gene expression is closely related to type and growth of muscle fibers .