SCOT 标记分析抗菌核病油菜资源的遗传多样性

摘要

【目的】探索 SCOT 标记在油菜遗传多样性研究中的应用。【方法】以8份具有代表性的甘蓝型油菜品种（系），对130个 SCOT 引物进行筛选。综合考虑多态性的大小，条带的清晰度，筛选出40个 SCOT 引物作为核心引物，对43份在田间接种鉴定表现抗菌核病的油菜材料进行遗传多样性分析。【结果】SCOT 标记共检测到372个位点，每个引物可以检测到5～12个多态性位点，多态性信息含量值（PIC）介于0．25～0．99，平均值为0．65。供试材料的遗传相似系数集中在0．60～0．88之间。遗传相似度较高，亲缘关系较近。【结论】SCOT 标记可以用于油菜遗传多样性的研究。%Objective]The aim of the study was to explore the utilization of start codon targeted polymorphism (SCOT)molecular marker technique in genetic diversity assessment in Brassica na-pus.[Method]8 representative rape materials were used as templates and 130 SCOT marker primers were screened out.40 of them showed specific bands and highly polymorphism.The ge-netic diversity of 43 Sclerotinia sclerotiorum resistant rape materials that was identified in the field were analyzed.[Results]Total 372 alleles were verified using SCOT markers and?about approximately 5-12 alleles were detected by each SCOT marker.Genetic polymorphism informa-tion content of each SCOT locus was between 0.25 to 0.99,with a mean value of 0.65.The ge-netic similarity coefficient ranged from 0.60 to 0.88.Tenetic diversity level of these tea materials was relatively high in this study.[Conclusion]SCOT marker is suitable to explore the genetic di-versity of rape germplasm.