猪乙型脑炎病毒基因Ⅰ型弱毒株免疫小鼠后诱导INF-γ的能力

摘要

[目的]评估基因Ⅰ型猪乙型脑炎病毒弱毒株(SCYA201201株)作为候选疫苗诱发机体产生细胞免疫的能力.[方法]将基因Ⅰ型猪乙型脑炎弱毒株(SCYA201201株F122代次)腹腔免疫小鼠后使用ELISA检测试剂盒测定早期的INF-γ水平,之后测定INF-γ在小鼠体内的持续时间.[结果]结果显示:猪乙型脑炎SCYA201201弱毒株(F122代次)经腹腔免疫小鼠后,小鼠血清中的INF-γ在免疫后6 h就可上升到一个较高水平,且INF-γ在血清和小鼠脾脏淋巴细胞中均可持续14 d以上.[结论]猪乙型脑炎SCYA201201弱毒株(F122代次)免疫小鼠后能够在早期诱发INF-γ产生,并且能够维持14 d以上,有作为候选疫苗的潜力.%[Objective]To evaluate the ability of Japanese encephalitis virus SCYA201201 strain of the genotype Ⅰ to induce cellular immunity as candidate vaccine in a mouse model.[Method]This resea-rch used ELISA kit to detect the INF-γ levels of mice serum after immune with genotypeⅠof Japanese encephalitis live-attenuated strain (SCYA201201). [Result]The results showed that Japanese encep-halitis live-attenuated SCYA201201 strain (F122 generation) of mice can induce INF- γ level to a higher level after 6-hour immunization in mice serum,INF-γ in serum and lymphocytes were sustained for more than 14 days. [Conclusion]The study proved that the live-attenuated SCYA201201 strain can induce the production of INF-γ in the early immunity stage,and it can be maintained for a long time.