类透明质酸壳聚糖微乳和碳铂联合给药对大鼠胶质瘤的治疗效果评价

摘要

Objective: To study the therapeutic effect of hyaluronic acid chitosan⁃based microemulsion ( HAC⁃ME) and carboplatin in a Wistar rat model bearing cerebral C6 glioma xenografts. Methods:C6 rat glioma cells were cultured normally. A total of 30 Wistar rats bearing orthotopic C6 glioma xenografts were randomly divided into 3 groups, and administrated with physiological saline, carbopl⁃atin or HAC⁃ME and carboplatin in each group. There are 10 rats in each group. The rat apoptosis changes and survival time were ob⁃served after treated with physiological saline, carboplatin or HAC⁃ME and carboplatin in each group. Results:Glioma cells were nega⁃tive in saline group with TUNEL staining, the nuclei of individual glioma cells in glioma tissue were stained with brown, indicating ap⁃optosis occur in carboplatin group, the apoptosis of glioma cells in glioma tissue significantly increased in HAC⁃ME and carboplatin group with TUNEL staining. The survival time in HAC⁃ME and carboplatin group was longer than that in saline group and carboplatin group ( P<0�05) . Conclusion:Administration of HAC⁃ME and carboplatin can suppress the growth of C6 glioma in rats and may pro⁃vide experimental basis for clinical treatment of glioma.%目的：制备类透明质酸壳聚糖微乳（ hyaluronic acid chitosan⁃based microemulsion， HAC⁃ME）和碳铂药物组合，并评价药物对C6大鼠胶质瘤动物模型的治疗疗效。方法：常规培养C6大鼠胶质瘤细胞，制备大鼠脑胶质瘤模型动物30只；随机分为生理盐水组、碳铂组、 HAC⁃ME和碳铂联合用药组，每组10只。分别用生理盐水、碳铂、 HAC⁃ME和碳铂联合用药对大鼠C6胶质瘤模型治疗后，观察胶质瘤细胞的凋亡情况、大鼠的生存期。结果：生理盐水组胶质瘤细胞TUNEL染色阴性；碳铂组TUNEL染色可见胶质瘤组织中个别肿瘤细胞的细胞核染成棕褐色，说明个别胶质瘤细胞发生凋亡； HAC⁃ME和碳铂联合用药组胶质瘤组织中肿瘤细胞的凋亡发生明显增多。 HAC⁃ME和碳铂联合用药组生存期长于生理盐水组和碳铂组（ P＜0�05）。结论： HAC⁃ME和碳铂联合用药能抑制大鼠胶质瘤细胞的生长，为临床胶质瘤治疗提供实验依据。