用635nm半导体激光器作为激光源,探测牙齿激发后产生的荧光.诊断结果显示正常牙齿和牙结石都在663nm处产生了明显的荧光峰,但是牙结石在650 ～ 700nm的波段内的荧光强度明显高于正常牙组织,在635nm处反射光强度明显低于正常牙齿.根据此现象,利用荧光峰的区域面积(650 ～ 730nm)与反射光峰的区域面积(618～648nm)比值R来判定牙齿是否有牙结石,在统计学上存在显著差异,牙结石R值低于正常牙齿R值.为了最大程度模拟临床环境,研究了猪肉组织对诊断结果的影响,结果表明在牙结石表面覆盖猪皮组织后,诊断的灵敏度和准确度仍高达90％以上.荧光诊断是一种无损诊断方法,本研究可为临床诊断提供重要的参考数据.%A diode laser with wavelength at 635 nm was selected as excitation light source. The teeth samples were excited by the light source and corresponding fluorescence spectra were collected. The detection results showed that fluorescence peak around 663 nm was observed for both normal teeth and dental calculus. However,for dental calculus,the fluorescence intensity between 650nm and 730nm was apparently higher than that of healthy tissue and the reflected light intensity at 635 nm was lower than that of normal teeth. According to this phenomena,the ratio of the fluorescence area (650～730 nm) and that of reflected light (618～648nm) was proposed to detect dental calculus. The value of R for dental calculus is much lower than that for normal teeth. To simulate the clinical environment, the detection area was covered with pig tissue. The value R was found to distinguish dental calculus with a sensitivity and specifity greater than 90%. This fluorescence detection has no damage to normal teeth and has potential for the detection of dental calculus.
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