玉米纹枯病菌UP-PCR体系的建立及遗传多样性的分析

摘要

Essential factors affecting the results of UP-PCR (Universally Primed PCR) analysis were tested and compared. An optimal reaction system suited for UP-PCR in genetic diversity was established. Seven polymorphic primers were selected to study the genetic diversity among 16 standard-isolates and 22 tester isolates coming from the major com planting areas in Northeastern China. The results showed that 105 amplified loci were detected, of which 102 (97.1%) were polymorphic. The isolates were clustered into 10 groups at the similar coefficient 0.685 by cluster analysis, of which, all the isolates belong to AG1-IA were clustered into one group. Cluster analysis showed that there was a clear correlation between genetic diversity and anastomosis group, but no significant correlation between genetic diversity and geographic source.%对UP-PCR(Universally Primed PCR)反应中的一些重要参数进行了优化,建立适合于玉米纹枯病菌的UP-PCR反应体系.对分离自辽宁、吉林、黑龙江等玉米主产区的22个菌株和16个标准菌株进行遗传多态性分析,筛选出7个扩增多态性好且稳定的通用引物,共扩增出105条带,其中多态性条带102条,占总条带数的97.1％.当相似系数为0.685时,可将38株镰孢菌菌株划分为10个类群,其中所有AGl-IA菌株聚为一个类群.聚类分析结果表明,供试菌株的遗传多样性与其所属融合群有着明显的相关性,而与其地理来源之间无明显的相关性.