为提高黑龙江省寒地粳稻稻瘟病抗性，通过杂交、回交、自交选育和分子标记辅助选择技术，将籼稻品种谷梅4号的广谱抗稻瘟病基因Pigm导入到寒地优良粳稻品种空育131、龙粳26和垦鉴稻6中，获得12个携带Pigm基因的优良导入系。与受体亲本相比，各导入系对稻瘟病菌的抗谱大幅提高，抗性频率最高达98.4%，最低为95.2%。同时对穗颈瘟的抗性也显著提高，导入Pigm基因后空育131穗颈瘟抗性由7级提高到1级，龙粳26穗颈瘟由5级提高到0~1级，垦鉴稻6穗颈瘟由7级提高到1~3级。结果还表明Pigm基因的导入影响株高和产量性状，其表现为株高增加，穗粒数增加，结实率下降，其中5个导入系产量显著提高。综合抗病性和产量性状可知，这5个Pigm基因的导入系可用于抗稻瘟病育种中。%In order to improve the blast resistance in japonica rice of Heilongjiang province, through marker-assisted selection (MAS), the broad-spectrum blast resistant gene Pigm from Gumei 4 (indica) was successfully introgressed into Kongyu 131, Longjing 26 and Kenjiandao 6. Through cross, backcross and self-pollination breeding, 12 lines containing Pigm gene introgression were obtained. Compared with recipient parent, the blast resistant introgression lines were greatly improved, with resistant frequency up to 98.4% and the minimum of 95.2%. At the same time, panicle blast resistance was also improved by the introgessed Pigm gene. Kongyu 131 panicle blast resistance changed from 7 to 1 level, Longjing 26 from 5 to 0-1 level, Kenjiandao 6 from 7 to 1-3 level. The yields of all Pigm gene introgression lines also changed. The plant height and grain number per panicle increased, but seed setting rate became lower. Among these introgression lines, the yields of 5 lines were improved, and these lines could be applied in rice blast resistant breeding.
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