IL-8基因多态性对慢性HBV感染者血清IL-8水平的影响

摘要

目的 探讨IL-8基因多态性对血清IL-8水平的影响以及IL-8基因多态性在慢性HBV感染发病机制中的可能作用.方法 采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)检测60例慢性HBV感染者和60例健康人群IL-8-251A/T位点多态性,同时采用酶联免疫吸附试验(ELISA)检测各组血清中IL-8的水平.结果 慢性HBV感染者和健康对照者间 IL-8-251A/T多态性位点各基因型频率和等位基因频率相比,差异有统计学意义(P<0.05).基因型为AA型、TA型的慢性HBV感染者其血清IL-8水平显著高于基因型为TT型的慢性HBV感染者(P<0.01)和所有基因型的健康对照(P<0.01).结论 IL-8-251A/T基因多态性与慢性HBV感染者血清IL-8水平有密切关系,提示IL-8-251A/T基因多态性可能会影响慢性HBV感染中患者的转归起重要的作用.%Objective To explore the effect of IL-8 gene polymorphism on levels of serum IL-8 in patients with chronic HBV and its role in the mechanism of the chronic HBV infection. Methods Polymorphisms of IL-8 gene were typed using polymerase chain reaction-restriction fragment length polymorphism( PCR-RFLP ) in 60 patients with chronic HBV and 60 healthy controls. The serum levels of IL-8 were measured by enzyme-linked immunosorbent assay( ELISA ). Results There was significant difference in frequencies of allele and genotype in IL-8 - 251A/T polymorphism between patients with chronic HBV infection and healthy controls( P < 0.05 ). The levels of serum IL-8 in HBV patients with genotypes AA and TA were significantly higher than those of HBV patients with genetype TT ( P <0.01 ), and the healthy controls with genetypes TT, AA,TA( P <0. 01 ). Conclusion The results suggest that the IL-8 gene polymorphism may play an important role in the mechanism of the chronic HBV infection.