提高大白菜小孢子植株试管苗移栽成活率的研究

摘要

为提高大白菜小孢子植株试管苗移栽的成活率,对影响其成活率的水质、栽培方式、基质、栽培时间4个因素进行了研究.结果表明:移栽时用蒸馏水浇灌,成活率达87.33％；根部带培养基移栽,成活率达93.67％; 12月份移栽成活率达80％；介质使用纯腐殖质移栽成活率最高,达100％.大白菜小孢子植株试管苗最好在12月份、以纯腐殖质作为基质、根部带培养基、用蒸馏水浇灌,移栽成活率最为理想.%The survival rate of transplanting plantlet in vitro in isolated-microspore culture of Chinese cabbage were affected by water quality, cultivation technology, matrix and culture time. The results showed that the survival rate of transplanting of mi-crospore plantlet of Chinese cabbage in vitro was 87.33% by using distilled water, 93.67% by roots with medium, 80% by transplanting in December, 100% by using hypertension as matrix. In a word, the best transplanting method is using distilled water and hypertension as matrix, roots with medium, in December.