抗白粉病小偃麦衍生品系高分子量麦谷蛋白亚基分析

摘要

High-molecular-weight glutenin subunit compositions of 64 wheat varieties powdery mildew resistance that derived from Octoploed Trititrigia were identified and analysed through SDS-PAGE technology. Studies showed that there were extremely plentiful HMW-GS types in these tested materials. 3, 5 and 2 kinds of subunit types were tested at Glu-A 1, Glu-B1 and Glu-D1 loci respectively. The majority of varieties have null-subunit at Glu-A1 loci among them, which accounts for 90% at this loci; Variation types are the most plentiful at Glu-B1 loci, the frequency of 7 + 8-subunit is the highest, which is 50%; The frequency of inferior 2 + 12-subunit is the highest, which is 78%; The frequency of superior 5 + 10-subunit that was known wildly is 22%. Therefore the wheat varieties that powdery mildew resistance derived from Octoploed Trititrigia are used to invent new germplasm to offer good parents for crop heredity breeding.%采用聚丙烯酰胺电泳技术(SDS-PAGE)分析了64个抗白粉病八倍体小偃麦衍生品系的高分子量麦谷蛋白亚基( HMW-GS)组成.结果表明,在供试材料中亚基组成类型极其丰富.在Glu-A1,Glu-B1和Glu-D1这3个位点上分别检测到3,5,2种不同的亚基组成类型.其中,在Glu-A1位点上大多数品种都具有null亚基,占该位点亚基的90％；在Glu-B1位点上的变异类型最丰富,7+8亚基出现频率最高,为50％；在Glu-D1位点上,劣质亚基2+12出现的频率最高,为78％,被世界公认的优质亚基5+10出现的频率为22％.这些抗白粉病小麦种质可为育种工作者提供优点突出而无突出缺点的亲本.