拟南芥温敏雄性不育突变体atms1的获得及表型分析

摘要

An ambient temperature Arabidopsis thaliana male sterile mutant ambient temperature-sensory male sterile 1 (atmsl), generated from ethylmethane sulfonate (EMS) mutagenesis has been identified. At low temperature 16 ℃, atmsl showed 100% pollen viability and silique fertility, while with the culture temperature increased, atmsl showed decreasing pollen viability and silique fertility. Therefore atmsl is a temperature sensory male sterile mutant. The morphological assay demonstrated that atmsl anther showed few significant impairments compared with wild-type anther when ambient temperature was below 23 ℃. At higher temperature 27 ℃ , the atmsl anther exhibited pleiotropic phenotypes: the developmental phases of anthers within one flower differed significantly; the callose layer of pollen mother cell of mutant was much thinner than that of wild-type, and the atmsl tapetum development exhibited much retarded than corresponding wild-type tapetum. Genetic assay demonstrated that the phenotypes of atmsl were controlled by a single recessive locus.%从甲基磺酸乙酯( ethylmethane sulfonate,EMS)化学诱变建立的野生型拟南芥(Col-0)突变体文库中筛选到1株突变体.在低温16℃时,该突变体的雄性育性与野生型没有显著差异,花粉染色呈现100％可育.随着培养环境温度的升高,突变体花粉育性逐渐下降,因此,该突变体为一温敏雄性不育突变体,并被命名为atms1(ambient temperature-sensory male sterility 1,即环境温度敏感雄性不育1).花药切片结果显示,在23℃以下,该突变体花药各个发育时期的形态与野生型花药没有显著的差异；而27℃处理1周后的突变体花药呈现多种表型:同一朵花中各个花药的发育时期出现显著分化,花粉母细胞胼胝体单薄,绒毡层发育滞后于同时期的野生型花药.遗传分析确定,atms1的不育表型是由单个隐性核基因控制的.