NADH在纳米金/过氧化聚吡咯复合材料修饰电极上的电催化氧化

摘要

A novel biosensor was fabricated by electrochemical deposition of gold nanoclusters on overoxidized polypyrrole (PPyox) film, formed a nano-Au/PPyox composite on glassy carbon electrode (nano-Au/PPyox/GCE). The properties of the composite have been characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). The nano-Au/PPyox/GCE had strongly catalytic activity toward the oxidation of nicotinamide adenine dinucleotide (NADH). The catalytic peak currents increased linearly with increasing NADH concentrations in the range of 2.0×10−7 –1.2×10−3 mol/L with a detection limit of 5.0×10−8. In addition, the composite modified electrode has been applied to determination of NADH with satisfactory results.%采用电沉积技术在过氧化聚吡咯膜上制备纳米金,通过扫描电镜和X-射线光电子能谱对复合材料的形貌和结构进行表征。采用循环伏安和计时安培法研究烟酰胺腺嘌呤二核苷酸(Nicotinamide Adenine Dinucleotide, NADH)在纳米金/过氧化聚吡咯复合材料修饰玻碳电极上的电化学催化氧化反应。结果表明,复合材料修饰电极显著降低了NADH的氧化峰电位,峰电流与其浓度在2.0×10−7∼1.2×10−3 mol/L范围内呈现很好的线性关系,检测限为5.0×10−8 mol/L,该修饰电极可用于对NADH的线性检测。