The study on three-dimensional reconstruction of endometrium in vitro

摘要

Objectives:To establish a three-dimensional model of endometrium in vitro.Methods: The endometrial cells were cultured on a biodegradable hybrid Collagen-PGLA polymer mesh and observed by scanning electron microscopy and light micro-scopy.Results:It showed that the endometrial cells adhered and spread well on the surfacesof the collagen sponge of the hybrid mesh after being cultured for 5 days. After 2weeks, the cells proliferated to become confluence achieving a three dimensional struc-ture.Conclusion: The three-dimensional model of endometrium could be establishedusing the hybrid polymer mesh as a skeleton in vitro.