Sequence alterations of the whole mitochondrial genome in primary and recurrent ovarian carcinomas

摘要

Objectives:To investigate mitochondrial DNA(mtDNA)alterations in primary and recurrent ovarian carcinomas to illuminate the impact of chemotherapy on mtDNA.Methods:Complete mtDNA genomes of tumor tissue from 7 primary ovarian carcinoma patients without treatment and 9 recurrent ones with prior chemotherapies were sequenced as well as their matched normal tissue.MtDNA alterations,including somatic mutations and new polymorphisms and consequent amino-acid alterations were compared between the two groups.Results:A large number of mtDNA new polymorphisms(69)and somatic mutations(17)were found in 16 ovarian carcinoma samples.Chemotherapy might not lead to more,heteroplasmic mutations and consequent amino-acid alterations(P>0.05)in the recurrent ovarian carcinoma patients than in the untreated ones.Conclusions:MtDNA damage was not so certainly made by chemotherapies and some of the mtDNA defects might be part of the disease process rather than a consequence of treatment.