含雌激素的冷冻保护剂对少弱精子玻璃化冻存效果的研究

摘要

Objective: To compare the effect of different cryoprotective mediums (CPMs) containing estrogen on the sperm vitrified cryopreservation in patients with oligoasthenozoospermia.Methods: The different cryoprotective-mediums containing estrogen were applied to forty infertile men with oligoastenozoospermic azoospermia.The viability, motility, morphology and spermatozoa hypoosmotic swelling rate (HOSR) of the infertile man sperm were compared before and after cryopreservation and between the study groups.Results: There are differences in vitrified effects during using four kinds of CPMs.The viability, motility, morphology and HOSR of sperm were significantly decreased after thawing (P＜0.05), but the viability and motility of the sperm were improved by using CPMs containing glycerol +E2 and GYC+ E2.Conclusions: Vitrified cryopreservation inflicts obvious damage on sperm.Estrogen is a feasible sperm cryoprotectant.%目的 比较添加雌激素的冷冻保护剂对少弱精子的冻存效果.方法 将40例少弱精子患者的精液标本分别采用甘油、甘油-卵黄-柠檬酸钠(GYC)、甘油+雌激素(甘油+ E2)、甘油-卵黄-柠檬酸钠+雌激素(GYC+ E2)四种冷冻保护剂(CPM)冷冻,比较冻融前后精子活率、活力(a+b级)、正常精子形态百分率、精尾低渗肿胀率(HOSR).结果 四种CPM对少弱精子的冷冻效果存在差别,精子活率、活力、正常形态百分率、精子尾部低渗肿胀率在冻后都有不同程度下降(P<0.05),但甘油+E2、GYC+ E2 能显著提高冻存后精子的活率及活力(a+b级).结论玻璃化冻存对精子存在损伤,含雌激素的冷冻保护剂可以提高低质量精液的冻存效果.