不同来源精子行卵胞浆内单精子注射临床结局的分析

摘要

Objective: To analyze the clinic outcome of using different sources of sperm for intracytoplasmic sperm injection (ICSI)and observe if sperm maturity affects the outcome of ICSI.rnMethods: The data of 227 cycles with ICSI treatment in our reproductive center from Jan. 2009 to Dec. 2011 were retrospectively analyzed. These cycles were divided to four groups according to the sperm sources: 17 cycles with sperm from ejaculated semen of failure of half intracytoplasmic sperm injection (half-ICSI) , 113 cycles with sperm from the patients with oligo-asteno-teratozoospermia, 76 cycles with spermatozoa from percutaneous puncture epididymis, and 21 cycles with spermatozoa from testicular sperm extraction. The rates of fertilization, cleavage, high quality embryo, implantation and pregnancy were compared among the groups respectively.rnResults: The rates of fertilization, cleavage, high quality embryo, implantation and pregnancy were not significant among the four groups (P>0. 05).rnConclusions: The clinical outcomes among the different groups using the different source of sperm for ICSI are similar. The sperm maturity might not affect ICSI clinical outcome.%目的 对采用不同来源的精子行卵胞浆内单精子注射(ICSI)的临床结局进行分析,以观察精子的成熟度是否影响ICSI的临床结局.方法 对本中心2009年1月至2011年12月227个ICSI治疗周期的结局进行回顾性分析.227个周期按精子来源分为4组:体外受精完全失败的射精液精子行部分卵母细胞ICSI(half-ICSI)17周期;重度少弱畸精子组113周期;经皮附睾穿刺取精子(PESA) 组76周期;以及睾丸穿刺取精子(TESA)组21周期.比较各组间的受精率、卵裂率、优质胚胎率、种植率以及妊娠率等各项指标.结果 四组ICSI治疗后的受精率、卵裂率、优质胚胎率、种植率及妊娠率均无统计学差异(P>0.05).结论 用不同来源精子进行ICSI治疗的临床效果相似,精子的成熟度可能不会影响ICSI的临床结局.