人工卵母细胞激活在ICSI受精失败周期中的应用

摘要

在辅助生殖技术领域,人工卵母细胞激活(AOA)是解决单精子卵胞浆内显微注射(ICSI)低受精或完全未受精周期中精子缺陷所致卵母细胞激活失败的重要措施.文献报道AOA的方法包括机械刺激、电刺激和化学激活.但与传统ICSI相比,ICSI联合AOA(ICSI-AOA)对ICSI受精失败周期的有效性和安全性尚难以评估.本文就AOA在ICSI受精失败周期中的应用,包括卵母细胞激活的机制、ICSI受精失败原因、AOA的方法和AOA的有效性及安全性进行综述,并探讨AOA在临床应用中存在的问题,以期更好地服务于临床.%In the field of assisted reproductive technology,artifical oocyte activation (AOA)is an important treatment method in ICSI cycle with previous low fertilization rate or total fertilization failure due to oocyte activation failure caused by sperm deficiency.Several methods of AOA including mechanical stimulation,electrical stimulation and chemical activation have been reported.However,it is difficult to assess the clinical efficacy and safety of ICSI with AOA compared with conventional ICSI in the couples of previous fertilization failure.The present article reviews the clinical practice of AOA in ICSI cycle with previous fertilization failure,the mechanism of oocyte activation,and the influent factors of ICSI fertilization failure,as well as AOA methods and the efficacy and safety of AOA,in order to explore the current problems of the clinical practice of AOA and better serve clinical application.