IFN-γ基因多态性与内蒙古汉族IgA肾病易感性关系的研究

摘要

目的:探讨IFN-γ基因+874位点单核苷酸多态性与内蒙古汉族IgA肾病患者易感性的关系.方法:从131例内蒙古汉族IgA肾病的患者和138例健康对照者外周血中提取DNA,采用序列特异性引物聚合酶链反应(PCR-SSP)技术检测IFN-γ基因+874位点单核苷酸多态性.比较两组的基因型和等位基因的分布.结果:IgA肾病组中IFN-γ基因+874位点AA基因型频率和A等位基因频率显著高于正常对照组(P＜0.05).结论:IFN-γ基因+874位点单核苷酸多态性可能是IgA肾病的易感因素.%Objective To Study the Relationship between the single nucleotide polymorphism ( SNP ) in IFN-γ+874 and IgA nephropathic susceptibility of the Han nationality patients in Inner Mongolia autonomous region. Methods Extract DNA from one hundred and thirty-one IgA nephropathy patients and 138 normal controls. The SNP in IFNγ+874 gene were determined by special sequence primer of polymerase chain reaction ( PCR-SSP ). IFNγ+874 genetype and allele frequency were compared between patients with IgA nephropathy and normal controls. Results A significant difference of genetype and allele distribution in IFN-γ+874 have been found between the patients with IgA nephropathy and healthy controls. Conclusion The SNP in IFNγ + 874 may be the predisposing factor for IgA nephropathy.