Activin A is a member of TGF-β superfamily and plays important roles in the development of animal.The strikingly action was the promotion of FSH secretion to regulate the growth of follicles.Activin can bind to the receptor in the cell surface and regulate the proliferation,differentiation and apoptosis through the way of autocrine / paracrine and internal secretion.The aim of this study is to verify whether Activin could increase the ratio of germ cells survival and development in vitro culture and promote the germ cells grow into oocytes.As in vitro culture of the 12.5 day post coitum (dpc) female embryonic gonads exposed to Activin A(100ng/mL).We found that Activin A is in favor of the female primordial germ cells to maintain the early properties and the differentiation into oocyte.The expression of Cx43 and Zp3 became higher in Activin group when detected by RT-PCR.Taken together,our results suggest that oocyte quality is enhanced by Activin A.%激活素A是TGF-β超家族中的成员之一,在整个动物机体发育过程中扮演重要角色,尤以促进FSH分泌来调节卵泡的发育.激活素A是一种多功能细胞因子,以自分泌/旁分泌和内分泌的方式,通过细胞表面的受体信号转导途径调控细胞的增殖、分化和凋亡.本实验的目的是检测激活素A能促进原始生殖细胞在体外培养条件下的存活和发育,并促进原始生殖细胞发育成卵母细胞.结果表明:培养12.5dpc雌性胎鼠生殖嵴时添加100ng/mL激活素A,可促进早期生殖细胞特征的保持,促进原始生殖细胞向卵母细胞分化.利用荧光定量RT-PCR检测了Cx43和Zp3的表达,结果发现激活素A可提高卵母细胞质量.
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