Objective:To fabricate bone tissue that has sim ilar structural and mec hanical characters with normal bone.Methods: Titanium meshes were molded into the shape of column in the length of 12 mm and in the diameter of 8 mm. The column was filled with natural coral granduls.4× 107 marrow deriv ed os teoblasts in 200 μl cell culture medium were seeded into each of f ive scaffolds and incubated in vitro for 2 d to ensure that cells adhere well on the s caff olds. Then the scaffolds were implanted subcutaneously into the back of nude mic e. T wo months after implantation, the animals were sacrificed and the implanted mate rials were investigated by gross specimen inspection , X-ray examination and histological observation. Results:2 months after in viv o incubation, the newly formed tissue was red and had the gross appearance of bo ne, and kept the original shape of column. Titanium mesh situated in the surface area. X-ray examination showed that large amount of new bone formed in the sca f folds, there was no space between new bone and titanium mesh. Most of co ral granduls had been absorbed. Histological observation demonstrated that in th e surface area, new bone integrated well with titanium mesh and was enforced by titanium mesh(like cortical bone), and in the middle area large amount of l amellar bone formed.Conclusion: Newly formed bone in this experiment has simil ar structural with normal cortical bone.%目的：建造用钛网增强的组织工程骨，使其在结构、力学特性上更接近正常骨组织，以满足承力部位植骨的需要。方法：将钛网制备成直径8 mm，长12 mm的圆柱状，填入直径2～3 mm的珊瑚颗粒，成为复合支架材料。在复合支架中植入骨髓来源的成骨细胞，体外继续培养2 d后，植入裸鼠背部皮下组织中，2个月后取材。通过大体标本观察、X线片检查、磨片的组织学检查，观察支架中新骨的形成情况及与钛网的愈合情况。结果：大体标本观察，新形成的组织为均匀的暗红色，表面光滑，钛网位于其中；X线片检查表明复合支架中有大量X线阻射影，均匀一致，与钛网间无间隙；磨片的组织学检查证实，在支架中有大量成熟的新骨形成，并与钛网愈合良好。结论：钛网可与组织工程骨相互愈合，用于承力部位植骨，将有广阔的应用前景。
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