根管冲洗剂次氯酸钠和氯亚明的细胞毒性

摘要

Objective:To evaluate the cytotoxicity of sodium hypochlorite(NaOCl) and chloramine-T at a same available chlorine concentration. Methods: L929 fibroblasts were used to test the cytotoxicity of NaOCl and chloramine-T at a same available chlorine concentration (0.500 0%, 0.250 0%,0.200 0%, 0.150 0%, 0.125 0%, 0.062 5%). Cell viability after 2 h, 30 min or 10 min exposure were measured by MTT assay. Results: Except the 0.500 0%-2 h group and 0.062 5%-30 min group, cell viability of NaOCl groups were higher than chloramine-T group(P<0.05). Conclusion: NaOCl solution has less cytotoxicity than chloramine-T at a same available chlorine concentration lower than 0.250 0%.%目的:评价次氯酸钠(NaOCl)和氯亚明溶液在相同有效氯浓度下的细胞毒性.方法: 按有效氯浓度为0.500 0%、0.250 0%、0.200 0%、0.150 0%、0.125 0%、0.062 5%分别配制NaOCl溶液和氯亚明溶液,使其与L929细胞接触2 h、30 min、10 min,用MTT法检测各组的细胞存活率.结果: 在所设定的浓度范围内,除高低两端组(0.500 0%-2 h及0.062 5%-30 min)外,细胞在其余各组NaOCl溶液中的存活率均大于氯亚明,差异有统计学意义(P<0.05).结论: 在0.250 0%以下的有效氯浓度范围,NaOCl溶液的细胞毒性小于氯亚明溶液.