目的:通过检测子宫腺肌病组织芯片中雌激素受体(ER)、孕激素受体(PR)、类固醇受体辅助活化因子-1(SRC-1)和核受体辅阻遏子(NCoR)的表达,观察SRC-1、NCoR与ER、PR之间的关系,探讨其在子宫腺肌病发生、发展中的作用.方法:用组织芯片技术和免疫组化方法检测42例子宫腺肌病患者异位子宫内膜组织(异位内膜组)和15例因子宫肌瘤行子宫切除术患者非瘤区对照子宫内膜组织(对照内膜组)中ER、PR、SRC-1和NCoR的阳性细胞的平均光密度值(IOD),及两组子宫内膜组织的增殖期和分泌期的IOD值,并进行比较.结果:ER、PR在异位内膜组中的IOD值显著低于对照内膜组(P＜0.05)；SRC-1在异位内膜组中IOD值明显高于对照内膜组(P＜0.05)；NCoR在异位内膜组中IOD值明显低于对照内膜组(P＜0.05)；异位内膜组中SRC-1、NCoR、ER和PR的IOD值增殖期和分泌期的比较,差异无统计学意义(P＞0.05)；对照内膜组中SRC-1、NCoR、ER和PR的IOD值增殖期高于分泌期(P＜0.05).结论:低表达的ER存在时,SRC-1在异位内膜组织中表达增高,低表达的PR可能降低NCoR的表达,SRC和NCoR可能导致子宫腺肌病异位内膜组织增生活性增高,可能与子宫腺肌病的种植和侵袭有关.%Objective:To explore the role of ER, PR, SRC-1 and NCoR in the development of adenomye-sis (Am)and the relationship of ER,PR and SRC-1, NCoR. Methods:The mean IOD values of cells with positive expression of ER, PR, SRC-1 and NCoR were detected by immunohistochemistry and tissue mi-croarray methods in 42 cases of ectopic endometria got in adenomyosis specimens (EE group) and 15 cases of normal endometria got in leiomyoma specimen (NE group), also the IOD values of endometrium in prolif-erative and secretive phases between the two groups were compared. Results: The IOD values of ER and PR in EE group were significantly lower than those in NE group (P<0. 05); The IOD value of SRC-1 was significantly higher in EE group than that in NE group( P<0.05) ; The IOD value of NCoR was significantly lower in EE group than that in NE group( P<0.05). The IOD values of ER, PR, SRC-1 and NCoR in EE group were no statistically significant differences between proliferative phase and secretory phase (P > 0.05); The IOD values of ER, PR, SRC-1 and NCoR in NE group in the proliferative phase were higher than those in secretory phase(P<0. 05). Conclusions:In the presence of low expression of ER, the expression of SRC-1 is strengthened in ectopic endometria, while the low expression of PR may reduce the expression of NCoR . The increased expression of SRC-1 and the decreased expression of NCoR may be involved in the implantation and infiltration of adenomyosis.
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