精原细胞瘤中M2A、AP-2γ、OCT3/4、PLAP和CD117的表达及其意义

摘要

目的:检测精原细胞瘤中M2A、AP-2γ、OCT3/4、PLAP和CD117的表达情况,探讨这些标记物在该肿瘤病理诊断及鉴别诊断中的意义.方法:采用免疫组化SP法,检测40例睾丸精原细胞瘤中M2A、AP-2γ、OCT3/4、PLAP和CD117的表达情况.结果:M2A、AP-2γ、OCT3/4 、PLAP和CD117阳性表达率分别为95.0 %、87.5 %、72.5%、67.5 %和75.0%,在13例PLAP表达阴性的精原细胞瘤中M2A和AP-2γ的阳性表达率分别为84.6%和76.9%.M2A、AP-2γ表达与OCT3/4、PLAP、CD117的表达比较,差异有显著性;M2A与 AP-2γ表达之间比较,差异无显著性,OCT3/4、PLAP与CD117间比较,差异也无显著性.结论:精原细胞瘤中M2A、AP-2γ高水平表达,可以作为诊断精原细胞瘤、特别是PLAP标记阴性的精原细胞瘤的免疫组化标记物,可作为鉴别诊断的有用指标.%Objective To detect the expressions of M2A, AP-2γ, OCT3/4, PLAP, and CD117, and to investigate the significances of these markers in pathologic diagnosis and differential diagnosis ,in semmomas.Methods The expressions of M2A, AP-2γ, OCT3/4, PLAP, and CD117 in 40 cases of' semmomas were investigated by immunohistochemical staining. Results The expression rates of M2A, AP-2γ, OCT3/4, PLAP,and CD117 were 95.0％ , 87.5％, 72.5％, 67.5％, and 75％, respectively. The expression rates of M2A and AP-2γ in 13 cases of PLAP-negative semmomas were 84.6％ and 76.9％ , respectively. There were significant differences among the expressions of M2A , AP-2γ and OCT3/4 , PLAP, CD117 , respectively. And there weren't significance differences between the expressions of M2A and AP-2γ , and between the expressions of OCT3/4 , PLAP and CD117 ,respectively. Conclusions M2A and AP-2γ are high expressed in seminomas, and may be used as immunohistochemical markers in diagnosing seminomas , especially when PLAP is negative. And may be an useful indicator for differentiating and diagnosing seminomas.