t-复合多肽1基因在原发性肾病综合征患者外周血单个核细胞中的表达

摘要

目的:初步探讨t-复合多肽1(TCP-1)基因在原发性肾病综合征(PNS)患者外周血单个核细胞(PBMC)中的表达及临床意义.方法:收集体检20例正常人(A组)和30例PNS患者(B + C组,其中B组为初发PNS患者,未用激素;C组为复诊PNS患者,使用激素2 ～ 3个月.各15例)的新鲜外周血液.用RT-PCR检测其外周血PBMC中TCP-1基因的表达.结果:A组分别与B + C组、B组、C组相比,TCP-1基因的表达差异均有显著性(P ＜ 0.01);B组与C组中的表达差异无显著性(P ＞ 0.05). 结论:TCP-1可能参与了PNS的发生发展过程.%Objective To explore the expression of t-complex polypeptide 1 (TCP-1) in patients with primary nephrotic syndrome (PNS) and its clinical significance. Methods Fresh blood samples were collected from 20 healthy aclults (group A) , and 30 PNS patients (group B + C) , 15 of whom received glucocorticoids for 2 to 3 months (group C) or no glucocorticoids (group B). The expressions of TCP-1 in peripheral blood mononuclear cells were detected hy RT-PCT. Results TCP-1 expression differed significantly between group A and group B + C (P ＜ 0.01), group B, or group C (P ＜0.01); however, there was no significant difference between group B and group C. Conclusions TCP-1 may be involved in the pathogenesis and development ofprimary nephrotic syndrome.