纳米氧化铁介导的mdr1反义寡核苷酸对人肠癌细胞的靶向投递作用

摘要

目的:以氧化铁磁性纳米颗粒(PLL-DCIONP)作为载体介导多药耐药基因1(mdr1)反义寡核苷酸(asODN)作用于人肠癌细胞,评价mdr1 asODN对人肠癌细胞的靶向投递作用.方法:荧光显微镜观察PLL-DCIONP-asODN-FAM复合物、asODN-FAM体外转染LS174T、HCT-8/VCR细胞,普鲁士蓝(Perle's blue)铁染色观察PLL-DCIONP-asODN复合物、PLL-DCIONP体外转染LS174T、HCT-8/VCR细胞,MRI测定体外转染细胞磁化率.结果:LS174T、HCT-8/VCR细胞在PLL-DCIONP-asODN-FAM复合物转染组荧光强度明显高于asODN-FAM转染组,LS174T、HCT-8/VCR细胞在PLL-DCIONP-asODN复合物转染组细胞内的蓝色铁颗粒明显多于PLL-DCIONP转染组,PLL-DCIONP-asODN复合物转染后的细胞T2随着细胞数的增加而逐渐缩短,呈明显的衰减势态.结论:PLL-DCIONP-asODN对HCT-8/VCR细胞有靶向投递作用.%Objective To study the Magnetic Iron Oxide Nanoparticles as a carrier-mediated MDR1 asODN on human colorectal Carcinoma cells, and evaluate the targeting delivery effect. Method LS174T、HCT-8/ VCR cultured with PLL-DCIONP-asODN-FAM、 asODN-FAM in vitro were observed under fluorescence microscope. LS174T、 HCT-8/VCR cultured with PLL-DCIONP-asODN、 PLL-DCIONP in vitro were observed by Perle's blue staining. In addition, the change of magnetic singal intensity was measured by 1.5T MRI. Results The fluorescence intensity of transfected PLL-DCIONP-asODN-FAM in LS174T, HCT-8/VCR cells was significantly higher than asODN-FAM transfection group. The blue iron particles of transfected PLL-DCIONP-asODN in LS174T, HCT-8/VCR cells were significantly more than PLL-DCIONP transfection group. After transfaction by PLL-DCIONP-asODN the cells spin-spin relaxitions were shortened gradually compared with increasing labeled cells and showed a trend to attenuation. Conclusions PLL-DCIONP-asODN has high targeting delivery effect on HCT-8/VCR.