初筛Rh（D）阴性无缘献血者弱D和Del表型调查

摘要

Objective To investigate the distribution of weak D and Del phenotype in unrelated blood donors with negative Rh (D). Methods Four hundred and nineRhD (-) unrelated donors were screened by the saline agglutination method. Type weak D was detected by the indirect antiglobulin test, and type Del was detected by absorb radiation method. Results In 409 RhD (-) samples, 27 cases (6.61%) of type weak D were checked out, and 61 cases (14.91%) were type Del and 321 cases (78.48%) were corroborate for RhD (-), In the confirmed RhD (-) blood donors, phenotype ccee was most (49.14%), followed by Ccee (23.47%), People with phenotype ccEe accounted for 4.16%in type weak D, followed by Ccee (1.71%). Ccee accounted for 10.02%in type Del, and Ccee accounted for 1.71%. Conclusion RhD (-) donors screened by regular testing should adopt a more sensitive test for verifing type weak D or Del. In order to ensure the security of blood transfusion, people with type weak D and Del should be regarded as RhD positive blood donors, and the RhD negative people deemed to be recipients.%目的：了解Rh（D）阴性献血者中弱D和Del表型的分布情况。方法：对初筛Rh（D）阴性无亲缘关系献血者采用间接抗球蛋白试验检测弱D型，采用吸收放散法检测Del型。结果：在409例盐水法初筛为Rh （D）阴性无亲缘关系献血者中，检出弱D表型27例，占6．61％，Del表型61例，占14．91％，确证Rh（D）阴性321例，占78．48％。在确证Rh（D）阴性献血者中ccee占49．14％，其次是Ccee，占23．47％；弱D表型中ccEe占4．16％，其次是Ccee，占1．71％；Del表型中Ccee占10．02％，CCee占1．71％。结论：对初筛Rh（D）阴性的献血者应采用更为敏感的试验确证是否为弱D表型或Del表型。为保障输血安全，弱D表型和Del表型献血者应作RhD阳性看待，而作为受血者则应视为RhD阴性。