阿托伐他汀对人CD4＋T淋巴细胞程序性细胞死亡因子4基因表达的影响

摘要

Objective To investigate the influence of atorvastatin (Lipitor) on the expression of programmed cell death 4 (PDCD4) in human CD4+T lymphocytes in vitro. Methods Human CD4+T cells obtained from healthy individuals were activated with PHA and treated with atorvastatin. The mRNA and protein expression levels of PDCD4 were detected by real-time PCR and western-blot respectively. Results The stimulation of PHA obviously increased the mRNA and protein expression of PDCD4 and the secretion of those serum cytokines. The expression of PDCD4 and the production of serum TNF-α were significantly decreased, whereas the serum levels of IL-10 were significantly increased after treated by different concentration of atorvastatin. The serum secretion of TNF-α was positive correlation with the expression of PDCD4 through the linear related analysis (r=0.782, P<0.01), and the secretion of IL-10 was negative correlation with the expression of PDCD4 (r=-0.653, P<0.05). Conclusion The anti-inflammatory effects of atorvastatin are mediated by down regulating the expression of PDCD4 in CD4+T cells.%目的：研究阿托伐他汀在体外对人CD4＋T淋巴细胞程序性细胞死亡因子4（PDCD4）表达的影响。方法：取20例健康志愿者的新鲜外周血，随机分为空白组、PHA刺激组、PHA＋（1、5、10μmol/L）阿托伐他汀组，体外培养48 h后进行PDCD4 mRNA和蛋白及相关炎性因子的检测。结果：PHA刺激组PDCD4 mRNA、蛋白表达及上清TNF-α及IL-10浓度均升高（均P＜0．05）；经阿托伐他汀干预后，能够使PDCD4 mRNA、蛋白表达量及TNF-α浓度明显下降，而上清液IL-10浓度进一步增加；TNF-α与PDCD4蛋白呈明显的正相关（r＝0．782，P＜0．01），IL-10与PDCD4蛋白呈明显的负相关（r＝-0．653，P＜0．05）。结论：阿托伐他汀能够通过调控人CD4＋T淋巴细胞PDCD4表达，从而发挥抗炎的作用。