肝复康对肝纤维化大鼠肝组织NF-κB、MMP-2和TIMP-2表达的影响

摘要

Objective To observe the effect of traditional Chinese medicine Gan-fu-kang (GFK)on hepatic expression of nuclear factor -κB (NF -κB),matrix metalloproteinase -2 (MMP -2)and tissue inhibitor of metalloproteinase -2 (TIMP-2)in carhon tetrachloride -induced liver fibrosis in rats. Methods SD rats were randomly divided into control,model,low,middle and high dose of GFK treatment groups.Liver fihrosis models of rats were made by subcutaneously injecting with 10％CCl4 for 12 weeks. The CFK was orally given daily with GJFK at concentration of 31.25 mg.kg. -1d-1,312.5 mg.kg. -1d -1 and 312.5mg.kg. -1d -1 from week 9 to 20. The expression of NF-κB protein and NF-κB,MMP-2 and TIMP-2 mRNA were detected by Western-blot or RT-PCR. Results Expression of NF-κB,MMP-2 and TIMP-2 markedly increased in model group and the administration of GFK could down-regulated expression of' these genes. Conclusion Gan-fu-kang has therapeutic effect on hepatic fibrosis. Its mechanism of action may be related to inhibition of the expression of NF-κB,MMP-2 and TIMP-2.%目的 研究中药肝复康对肝纤维化大鼠肝脏NF-κB、基质金属蛋白酶-2(MMP-2)和金属蛋白酶组织抑制因子2(TIMP-2)基因表达的影响.方法 随机将SD大鼠分为正常对照组、模型组、小剂量、中剂量和大剂量治疗组,制备CCl4肝纤维化模型,自第9周起,给予中药肝复康治疗至第20周.采用Western-Blot法检测NF-κB表达;采用RT-PCR法观察NF-κB、MMP-2和TIMP-2 mRNA水平.结果 模型组大鼠肝组织NF-κB、MMP-2和TIMP-2表达增加,肝复康可明显抑制上述因子的表达(P<0.01),且三者mRNA水平在各组间呈显著正相关.结论 肝复康治疗肝纤维化可能与其抑制NF-κB、MMP-2和TIMP-2的表达有关.