肝纤维化大鼠肝组织Raf/ERK信号通路的变化及肝复康的干预作用

摘要

目的 探讨中药肝复康通过Raf/ERK介导的信号转导通路治疗肝纤维化大鼠的作用机制.方法 制备四氯化碳诱导的肝纤维化大鼠,应用肝复康干预;采用RT-PCR法测定肝组织及HSC-T6细胞Raf、ERK和Ⅲ型胶原mRNA水平的变化;采用免疫组织化学法检测肝组织ERK的表达.结果 模型组肝组织Raf、ERK和Ⅲ型胶原mRNA水平较正常对照组明显上调(P<0.01),而肝复康治疗组则明显下调(P<0.01);模型组肝组织ERK蛋白的表达上调,而肝复康治疗组表达则下调.结论 肝复康对肝纤维化有明确的治疗作用,其抗肝纤维化的作用机制可能与ERK介导的信号转导通路有关.%Objective To investigate the mechanisms by which Gan-fu-kang inhibiting Raf/ERK signal transduction pathway in rats with hepatic fibrosis. Methods The rats with liver fibrosi were made by subcuta- neously injecting with 10%CCl4 and treated with Gan-fu-kang. Expression of Raf,ERK and collagen III Mrna were detected by RT-PCR. The ERK expression in liver tissues were detected by immunohistochemistry staining. Results The levels of Raf,ERK and collagen III Mrna in model group increased than in control group(P<0.01); In the immunohistochemistry staining, the expression of ERK protein in model group was obviously elevated than in Gan-fu-kang treatment group. Conclusion Ganfukang has effective treatment on liver fibrosis and its treatment mechanisms probably involved in ERK-induced signal transduction pathway.