中药肝复康对肝星状细胞mTOR/P70S6K信号通路的干预作用

摘要

目的 观察中药肝复康含药血清对乙醛刺激的肝星状细胞mTOR/P70S6K信号通路的影响,以探讨肝复康抗纤维化的分子生物学机制.方法 体外培养大鼠HSC-T6细胞,用肝复康对乙醛刺激的HSCs进行干预,采用RT-PCR法测定HSCs中Ⅰ、Ⅲ型胶原、mTOR、P70S6K的表达.结果 空白对照组肝星状细胞各指标表达量呈相对较低水平,乙醛刺激组细胞Ⅰ型胶原、mTOR、P70S6K 和Ⅲ型胶原表达量明显升高(P＜0.05),而肝复康干预对于细胞Ⅰ型胶原、mTOR和P70S6K的升高有明显的抑制作用(P＜0.01).结论 mTOR/P70S6K信号通路在HSCs活化过程中起到重要作用,肝复康发挥抗纤维化的作用机制与阻断mTOR/P70S6K信号通路有关.%Objective To study the effect of blood serum supplemented with Ganfukang compound; a traditional Chinese herb medicine; on the mTOR/P70S6K signal pathway in hepatic stellate cells (HSCs) .Methods HSC-T6 stimulated by acetaldehyde were incubated with blood serum of Ganfukang compound.The expressions of collagen- I , III , mTOR and P70S6K in each group were analyzed by RT-PCR. Results HSCs exhibited a low mRNA level of collagen- I , III > mTOR and P70S6K in the control group > whereas they were remarkably increased in the model group (P<0.01 or P<0.05);and the expression of them were downregulated after treated with blood serum of Ganfukang compound (P<0.01).Conclusions mTOR/P70S6K signal pathway play an important roles in HSCs activation; and the anti - fibrosis effects of Ganfukang are related to the inhibition of mTOR/P70S6K signal pathway.