邻苯二胺氧化反应体系中碳糊电极对H2O2的电位响应

摘要

A novel determining system based on the reaction between H2O2 and O-phenylenediamine（OPD） with the product of 2,3-diaminophenazine for detecting hydrogen peroxide（H2O2） by voltammetry was established and the potential response performance of carbon paste electrodes（CPEs） to H2O2 was investigated.Under optimum experimental conditions,CPEs showed the linear response range to H2O2 from 3.53×10^-6 to 6.38×10^-2mol·L^-1 and the lower detection limit was 1.91×10^-6mol·L^-1 and the slope of working curve was 23.42.The results indicated that in the designed system,carbon paste electrodes had low detection limit,high sensitivity,wide linear range,excellent electrode consistency and reproducibility.Compared with general electrochemical enzyme sensors,CPEs had simple preparation method,stable property,low cost of raw materials which were easy to obtain,and also had better application prospect.%基于H2O2氧化邻苯二胺（OPD）生成2,3-二氨基吩嗪反应,建立了一种电位法检测H2O2的新体系,研究了该体系中碳糊电极（CPE）对H2O2的响应性能.在优化实验条件下,CPE对H2O2的线性响应范围为3.53×10-6～6.38×10^-2 mol.L^-1,检测下限为1.91×10^-6 mol.L^-1,工作曲线斜率为23.42.结果表明,在所设计的新体系中,CPE检测下限低、灵敏度高、线性范围较宽,电极一致性和重现性较好.与一般的电化学酶传感器相比,碳糊电极制备方法简单、性能稳定、原料成本低且容易获得,具有较好的应用前景.